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water 0.2 g sodium azide (0.1%) TOXIC Mix together and store at 4°C Reuse solution to coat slides until it becomes noticeably less sticky Commercial poly-lysine slides (Sigma poly-prep) are not nearly as sticky as lab-made slides. Store at 4°C - kept in cold box Block should be made with serum from the same species used to generate the 2° Block must not contain serum from the animal used to generate the 1° We generally use secondary antibodies that were made by donkeys, so we use donkey serum (available from Jackson Immuno Research 800-367-5296) Filter solution through coarse filter back into bottle.If there is a tie, the second player will be asked a tie breaker question, and if they answer correctly, they will win the previously chosen crown.If they answer incorrectly, the first player gets the crown., Trivia Crack has bonus features or ‘power-ups’, such as extra spins, extra time (Phone a Friend), a double chance (Ask the Audience) or removing two of the four possible answers to help make things easier (50/50).Taxes and customs can be a hassle for new companies trying to enter China.To avoid the complexity of the matter, many companies get started with directly shipping parcels to end users, which prevents much of the red tape associated with registering a company and brand in China.